This case study explains how stakeholders in Cambodia effectively scaled up the provision of isoniazid preventive therapy (IPT) to prevent tuberculosis among people living with HIV. The publication covers the extent of tuberculosis and HIV in Cambodia, institutions collaborating to promote national scale-up and the role of research and global guidance in this effort. It also describes the development of national policies and frameworks to support the launch and scale-up of IPT and outlines facilitators and challenges in national implementation. The case study provides information for other countries interested in scaling up IPT.