This manual provides a standard approach to gender and human sexuality sensitivity awareness training for service providers from the USAID Open Doors project, implementing partners and the public and private sector. The focus is to build the capacity of service providers to provide friendly, nondiscriminatory and nonjudgmental HIV prevention, treatment and care services to members of key populations. The publication is also a reference manual on sexuality, gender and diversity issues as they relate to key populations in Zambia.