This manual provides a standard approach to gender and human sexuality sensitivity awareness training for service providers from the USAID Open Doors project, implementing partners and the public and private sector. The focus is to build the capacity of service providers to provide friendly, nondiscriminatory and nonjudgmental HIV prevention, treatment and care services to members of those at an increased risk for HIV. The publication is also a reference manual on sexuality, gender and diversity issues as they relate to those at an increased risk for HIV in Zambia.